Reporter

Part:BBa_K223046:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR - SoxS - GFP Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 672
    Illegal suffix found in sequence at 506
    Illegal SpeI site found at 757
    Illegal PstI site found at 771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal PstI site found at 521
    Illegal PstI site found at 771
    Illegal NotI site found at 27
    Illegal NotI site found at 514
    Illegal NotI site found at 678
    Illegal NotI site found at 764
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 507
    Illegal EcoRI site found at 672
    Illegal XbaI site found at 687
    Illegal SpeI site found at 757
    Illegal PstI site found at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 672
    Illegal XbaI site found at 36
    Illegal XbaI site found at 687
    Illegal SpeI site found at 507
    Illegal SpeI site found at 757
    Illegal PstI site found at 521
    Illegal PstI site found at 771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1446


Design Notes

Was tested on both a high and low copy plasmid.


Source

Stanford iGEM 2009

References